KIT | KIT-Bibliothek | Impressum | Datenschutz

Tracking emerging mycotoxins in food: development of an LC-MS/MS method for free and modified Alternaria toxins

Puntscher, Hannes; Kütt, Mary-Liis; Skrinjar, Philipp; Mikula, Hannes; Podlech, Joachim; Fröhlich, Johannes; Marko, Doris; Warth, Benedikt

Abstract:
Mycotoxins produced by Alternaria fungi are ubiquitous food contaminants, but analytical methods for generating comprehensive exposure data are rare. We describe the development of an LC-MS/MS method covering 17 toxins for investigating the natural occurrence of free and modified Alternaria toxins in tomato sauce, sunflower seed oil, and wheat flour. Target analytes included alternariol (AOH), AOH-3-glucoside, AOH-9-glucoside, AOH-3-sulfate, alternariol monomethyl ether (AME), AME-3-glucoside, AME-3-sulfate, altenuene, isoaltenuene, tenuazonic acid (TeA), tentoxin (TEN), altertoxin I and II, alterperylenol, stemphyltoxin III, altenusin, and altenuic acid III. Extensive optimization resulted in a time- and cost-effective sample preparation protocol and a chromatographic baseline separation of included isomers. Overall, adequate limits of detection (0.03–9 ng/g) and quantitation (0.6–18 ng/g), intermediate precision (9–44%), and relative recovery values (75–100%) were achieved. However, stemphyltoxin III, AOH-3-sulfate, AME-3-sulfate, altenusin, and altenuic acid III showed recoveries in wheat flour below 70%, while their performance was stable and reproducible. ... mehr

Open Access Logo


Verlagsausgabe §
DOI: 10.5445/IR/1000089500
Veröffentlicht am 15.01.2019
Originalveröffentlichung
DOI: 10.1007/s00216-018-1105-8
Scopus
Zitationen: 23
Web of Science
Zitationen: 22
Coverbild
Zugehörige Institution(en) am KIT Institut für Organische Chemie (IOC)
Publikationstyp Zeitschriftenaufsatz
Jahr 2018
Sprache Englisch
Identifikator ISSN: 1618-2642, 1618-2650
urn:nbn:de:swb:90-895002
KITopen-ID: 1000089500
Erschienen in Analytical and bioanalytical chemistry
Band 410
Heft 18
Seiten 4481–4494
Vorab online veröffentlicht am 16.05.2018
Schlagworte Alternaria alternata, Maskedmycotoxins, Perylenequinones, Liquidchromatography, Tandemmassspectrometry , Food safety
Nachgewiesen in Web of Science
Scopus
KIT – Die Forschungsuniversität in der Helmholtz-Gemeinschaft
KITopen Landing Page