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Serological Number for Characterization of Circulating Antibodies

Palermo, Andrea; Nesterov-Mueller, Alexander

Abstract:
The dissociation constant of the circulating IgG antibodies is suggested to be proportional to the partial concentrations of these antibodies in blood serum in equilibrium. This coefficient, called serological number, is a dimensionless parameter and may be equal for all antibodies in a serum. Based on the serological number, we derived the equilibrium equation of the humoral immune system which allows estimating the number of different binding motifs in a serum. This equation also allows estimating the number of binding motifs of posttranslational and conformational nature. The feasibility of measuring the serological number via peptide arrays was demonstrated. Fifteen peptides with unique binding motifs were incubated and stained with the blood serum of a healthy adult at different dilutions. From these experiments, the serological number was determined. The serological number may explain the pre-existing antibody response after vaccination.

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Verlagsausgabe §
DOI: 10.5445/IR/1000090548
Veröffentlicht am 06.02.2019
Coverbild
Zugehörige Institution(en) am KIT Institut für Mikrostrukturtechnik (IMT)
Publikationstyp Zeitschriftenaufsatz
Jahr 2019
Sprache Englisch
Identifikator ISSN: 1422-0067
urn:nbn:de:swb:90-905487
KITopen-ID: 1000090548
HGF-Programm 47.01.03 (POF III, LK 01)
Erschienen in International journal of molecular sciences
Band 20
Heft 3
Seiten Article: 604
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Vorab online veröffentlicht am 30.01.2019
Nachgewiesen in Web of Science
Scopus
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