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Self-Immobilizing Biocatalysts Maximize Space–Time Yields in Flow Reactors

Peschke, Theo; Bitterwolf, Patrick; Hansen, Silla; Gasmi, Jannis; Rabe, Kersten; Niemeyer, Christof

Abstract:
Maximizing space–time yields (STY) of biocatalytic flow processes is essential for the establishment of a circular biobased economy. We present a comparative study in which different biocatalytic flow reactor concepts were tested with the same enzyme, the (R)-selective alcohol dehydrogenase from Lactobacillus brevis (LbADH), that was used for stereoselective reduction of 5-nitrononane-2,8-dione. The LbADH contained a genetically encoded streptavidin (STV)-binding peptide to enable self-immobilization on STV-coated surfaces. The purified enzyme was immobilized by physisorption or chemisorption as monolayers on the flow channel walls, on magnetic microbeads in a packed-bed format, or as self-assembled all-enzyme hydrogels. Moreover, a multilayer biofilm with cytosolic-expressed LbADH served as a whole-cell biocatalyst. To enable cross-platform comparison, STY values were determined for the various reactor modules. While mono- and multilayer coatings of the reactor surface led to STY < 10, higher productivity was achieved with packed-bed reactors (STY ≈ 100) and the densely packed hydrogels (STY > 450). The latter modules could be operated for prolonged times (>6 days). ... mehr

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Verlagsausgabe §
DOI: 10.5445/IR/1000090920
Veröffentlicht am 12.02.2019
Originalveröffentlichung
DOI: 10.3390/catal9020164
Coverbild
Zugehörige Institution(en) am KIT Institut für Biologische Grenzflächen (IBG)
Publikationstyp Zeitschriftenaufsatz
Jahr 2019
Sprache Englisch
Identifikator ISSN: 2073-4344
urn:nbn:de:swb:90-909207
KITopen-ID: 1000090920
HGF-Programm 47.02.01 (POF III, LK 01)
Erschienen in Catalysts
Band 9
Heft 2
Seiten Article: 164
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Vorab online veröffentlicht am 08.02.2019
Schlagworte enzymes; flow biocatalysis; immobilization techniques; stereoselective reactions; biomaterials; micro reactors
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