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The RecQ-like helicase HRQ1 is involved in DNA crosslink repair in Arabidopsis in a common pathway with the Fanconi anemia-associated nuclease FAN1 and the postreplicative repair ATPase RAD5A

Röhrig, Sarah; Dorn, Annika; Enderle, Janina; Schindele, Angelina; Herrmann, Natalie J.; Knoll, Alexander; Puchta, Holger

Abstract:
RecQ helicases are important caretakers of genome stability and occur in varying copy numbers in different eukaryotes. Subsets of RecQ paralogs are involved in DNA crosslink (CL) repair. The orthologs of AtRECQ2, AtRECQ3 and AtHRQ1, HsWRN, DmRECQ5 and ScHRQ1 participate in CL repair in their respective organisms, and we aimed to define the function of these helicases for plants.
We obtained Arabidopsis mutants of the three RecQ helicases and determined their sensitivity against CL agents in single‐ and double‐mutant analyses.
Only Athrq1 , but not Atrecq2 and Atrecq3 , mutants proved to be sensitive to intra‐ and interstrand crosslinking agents. AtHRQ1 is specifically involved in the repair of replicative damage induced by CL agents. It shares pathways with the Fanconi anemia‐related endonuclease FAN1 but not with the endonuclease MUS81. Most surprisingly, AtHRQ1 is epistatic to the ATPase RAD5A for intra‐ as well as interstrand CL repair.
We conclude that, as in fungi, AtHRQ1 has a conserved function in DNA excision repair. Additionally, HRQ1 not only shares pathways with the Fanconi anemia repair factors, but in contrast to fungi also seems to act in a common pathway with postreplicative DNA repair.

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Verlagsausgabe §
DOI: 10.5445/IR/1000090926
Veröffentlicht am 29.05.2020
Originalveröffentlichung
DOI: 10.1111/nph.15109
Scopus
Zitationen: 9
Web of Science
Zitationen: 8
Cover der Publikation
Zugehörige Institution(en) am KIT Botanisches Institut und Botanischer Garten (BOTANIK)
Publikationstyp Zeitschriftenaufsatz
Publikationsmonat/-jahr 06.2018
Sprache Englisch
Identifikator ISSN: 0028-646X
KITopen-ID: 1000090926
Erschienen in The new phytologist
Verlag Wiley
Band 218
Heft 4
Seiten 1478–1490
Vorab online veröffentlicht am 25.03.2018
Nachgewiesen in Scopus
Web of Science
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