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The CRISPR/Cas revolution reaches the RNA world: Cas13, a new Swiss Army knife for plant biologists

Wolter, Felix; Puchta, Holger

Abstract:
Application of the bacterial CRISPR /Cas systems to eukaryotes is revolutionizing biology. Cas9 and Cas12 (previously called Cpf1) are widely used as DNA nucleases for inducing site‐specific DNA breaks for different kinds of genome engineering applications, and in their mutated forms as DNA ‐binding proteins to modify gene expression. Moreover, histone modifications, as well as cytosine methylation or base editing, were achieved with these systems in plants. Recently, with the discovery of the nuclease Cas13a (previously called C2c2), molecular biologists have obtained a system that enables sequence‐specific cleavage of single‐stranded RNA molecules. The latest experiments with this and also the alternative Cas13b system demonstrate that these proteins can be used in a similar manner in eukaryotes for RNA manipulation as Cas9 and Cas12 for DNA manipulations. The first application of Cas13a for post‐transcriptional regulation of gene expression in plants has been reported. Recent results show that the system is also applicable for combating viral infection in plants. As single‐stranded RNA viruses are by far the most abundant class of viruses in plants, the application of this system is of special promise for crops. ... mehr

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Verlagsausgabe §
DOI: 10.5445/IR/1000090977
Veröffentlicht am 29.05.2020
Originalveröffentlichung
DOI: 10.1111/tpj.13899
Scopus
Zitationen: 16
Web of Science
Zitationen: 13
Cover der Publikation
Zugehörige Institution(en) am KIT Botanisches Institut und Botanischer Garten (BOTANIK)
Publikationstyp Zeitschriftenaufsatz
Publikationsmonat/-jahr 06.2018
Sprache Englisch
Identifikator ISSN: 0960-7412
KITopen-ID: 1000090977
Erschienen in The plant journal
Band 94
Heft 5
Seiten 767–775
Projektinformation CRISBREED (EU, H2020, 741306)
Vorab online veröffentlicht am 04.05.2018
Nachgewiesen in Web of Science
Scopus
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