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Efficient Extraction from Mice Feces for NMR Metabolomics Measurements with Special Emphasis on SCFAs

Hauser, Adrian; Eisenmann, Philipp; Muhle-Goll, Claudia; Luy, Burkhard; Dötsch, Andreas; Graf, Daniela; Tzvetkova, Pavleta

Abstract:
Nuclear magnetic resonance (NMR) spectroscopy is one of the most promising methods for use in metabolomics studies as it is able to perform non targeted measurement of metabolites in a quantitative and non-destructive way. Sample preparation of liquid samples like urine or blood serum is comparatively easy in NMR metabolomics, because mainly buffer and chemical shift reference substance are added. For solid samples like feces suitable extraction protocols need to be defined as initial step, where the exact protocol depends on sample type and features. Focusing on short chain fatty acids (SCFAs) in mice feces, we describe here a set of extraction protocols developed with the aim to suppress changes in metabolite composition within 24 h after extraction. Feces are obtained from mice fed on either standard rodent diet or high fat diet. The protocols presented in this manuscript are straightforward for application, and successfully minimize residual bacterial and enzymatic activities. Additionally, they are able to minimize the lipid background originating from the high fat diet.

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Verlagsausgabe §
DOI: 10.5445/IR/1000093946
Veröffentlicht am 24.04.2019
Coverbild
Zugehörige Institution(en) am KIT Institut für Organische Chemie (IOC)
Institut für Biologische Grenzflächen (IBG)
Publikationstyp Zeitschriftenaufsatz
Jahr 2019
Sprache Englisch
Identifikator ISSN: 2218-1989
KITopen-ID: 1000093946
Erschienen in Metabolites
Band 9
Heft 3
Seiten Article: 55
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Vorab online veröffentlicht am 21.03.2019
Schlagworte metabolomics; mice; feces; faeces; NMR spectroscopy; short chain fatty acids; dietary fibers; extraction protocol; diet; NMR; extraction
Nachgewiesen in Web of Science
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