KIT | KIT-Bibliothek | Impressum | Datenschutz

Biofilm Bridges Forming Structural Networks on Patterned Lubricant‐Infused Surfaces

Lei, Wenxi; Bruchmann, Julia; Rüping, Jan Lars; Levkin, Pavel A.; Schwartz, Thomas

Abstract:
Despite many decades of research, biofilm architecture and spreading mechanisms are still not clear because of the heterogenous 3D structure within biofilms. Here, patterned “slippery” lubricant‐infused porous surfaces are utilized to study biofilm structure of Pseudomonas aeruginosa, Stenotrophomonas maltophilia, and Staphylococcus aureus. It is found that bacteria are able to spread over bacteria‐repellent lubricant‐infused regions by using a mechanism, termed “biofilm bridges”. Here, it is demonstrated that bacteria use bridges to form interconnected networks between distant biofilm colonies. Detailed structure of bridges shows a spatial distribution of bacteria with an accumulation of respiratory active bacteria and biomass in the bridges. The core–shell structure of bridges formed by two‐species mixed population is illustrated. It is demonstrated that eDNA and nutrients have a strong effect on biofilm bridges formation. Thus, it is believed that biofilm bridging is important to reveal the structure and communication within biofilms.

Open Access Logo


Verlagsausgabe §
DOI: 10.5445/IR/1000094417
Veröffentlicht am 10.07.2019
Originalveröffentlichung
DOI: 10.1002/advs.201900519
Coverbild
Zugehörige Institution(en) am KIT Institut für Organische Chemie (IOC)
Institut für Funktionelle Grenzflächen (IFG)
Institut für Toxikologie und Genetik (ITG)
Publikationstyp Zeitschriftenaufsatz
Jahr 2019
Sprache Englisch
Identifikator ISSN: 2198-3844, 2198-3844
KITopen-ID: 1000094417
HGF-Programm 47.02.06 (POF III, LK 01)
Erschienen in Advanced science
Band 6
Heft 13
Seiten 1900519
Vorab online veröffentlicht am 08.05.2019
KIT – Die Forschungsuniversität in der Helmholtz-Gemeinschaft
KITopen Landing Page