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Intercellular communication is required for trap formation in the nematode-trapping fungus Duddingtonia flagrans

Youssar, Loubna; Wernet, Valentin; Hensel, Nicole; Yu, Xi; Hildebrand, Heinz-Georg; Schreckenberger, Birgit; Kriegler, Marius; Hetzer, Birgit; Frankino, Phillip; Dillin, Andrew; Fischer, Reinhard

Abstract:
Nematode-trapping fungi (NTF) are a large and diverse group of fungi, which may switch from a saprotrophic to a predatory lifestyle if nematodes are present. Different fungi have developed different trapping devices, ranging from adhesive cells to constricting rings. After trapping, fungal hyphae penetrate the worm, secrete lytic enzymes and form a hyphal network inside the body. We sequenced the genome of Duddingtonia flagrans, a biotechnologically important NTF used to control nematode populations in fields. The 36.64 Mb genome encodes 9,927 putative proteins, among which are more than 638 predicted secreted proteins. Most secreted proteins are lytic enzymes, but more than 200 were classified as small secreted proteins (< 300 amino acids). 117 putative effector proteins were predicted, suggesting interkingdom communication during the colonization. As a first step to analyze the function of such proteins or other phenomena at the molecular level, we developed a transformation system, established the fluorescent proteins GFP and mCherry, adapted an assay to monitor protein secretion, and established gene-deletion protocols using homologous recombination or CRISPR/Cas9. ... mehr

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Verlagsausgabe §
DOI: 10.5445/IR/1000094564
Veröffentlicht am 14.05.2019
Originalveröffentlichung
DOI: 10.1371/journal.pgen.1008029
Scopus
Zitationen: 2
Coverbild
Zugehörige Institution(en) am KIT Institut für Angewandte Biowissenschaften (IAB)
Publikationstyp Zeitschriftenaufsatz
Jahr 2019
Sprache Englisch
Identifikator ISSN: 1553-7404
KITopen-ID: 1000094564
Erschienen in PLoS Genetics
Band 15
Heft 3
Seiten Article: e1008029
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Vorab online veröffentlicht am 27.03.2019
Nachgewiesen in Web of Science
Scopus
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