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Pulsed electric field (PEF)-assisted protein recovery from Chlorella vulgaris is mediated by an enzymatic process after cell death

Scherer, Daniel; Krust, Damaris; Frey, Wolfgang; Müller, Georg; Nick, Peter; Gusbeth, Christian

Abstract (englisch):
Pulsed electric field (PEF) treatment has so far been considered as ineffective for protein extraction, but the parameters that influence protein extraction Efficiency have not been identified fully. Using Chlorella vulgaris in this study, we show that up to half of the proteins present in the cell can be extracted via PEF treatment and
an incubation step for up to 24 h. The extraction efficiency is highly influenced by biomass concentration during the incubation step and the incubation temperature: in our experiments we were able to extract half of the proteins present in the cells at dilute concentrations (5 mg·ml−1), but this efficiency decreases with increasing biomass concentration, indicating that diffusion gradients play a role in the protein release. However, we also show that not only diffusion plays a role, but also a biological process that requires suitable conditions. We observed that PEF treated cells undergo programmed cell death, indicated by DNA laddering. Furthermore cold and hot temperatures as well as protease inhibition impair the release of proteins. In Western blots we can observe that some proteins are not released when the activity of proteases is blocked. ... mehr



Originalveröffentlichung
DOI: 10.1016/j.algal.2019.101536
Zugehörige Institution(en) am KIT Institut für Hochleistungsimpuls- und Mikrowellentechnik (IHM)
Publikationstyp Zeitschriftenaufsatz
Jahr 2019
Sprache Englisch
Identifikator ISSN: 2211-9264
KITopen-ID: 1000094795
HGF-Programm 35.13.01 (POF III, LK 01)
Erschienen in Algal Research
Band 41
Seiten Article no: 101536
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