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A Microcavity Array-Based 4D Cell Culture Platform

Nies, Cordula; Rubner, Tobias; Lorig, Hanna; Colditz, Vera; Seelmann, Helen; Müller, Andreas; Gottwald, Eric

(1) Background: We describe a 4D cell culture platform with which we tried to detect and to characterize migration dynamics of single hematopoietic stem cells in polymer film microcavity arrays integrated into a microtiter plate. (2) Methods: The system was set up with CD34-expressing KG-1a cells as a surrogate for hematopoietic stem cells. We then evaluated the system as an artificial hematopoietic stem cell niche model comprised of a co-culture of human hematopoietic stem cells from cord blood (cord blood CD34+ cells, hHSCs) and human mesenchymal stromal cells (hMSCs) from bone marrow over a period of 21 days. We used a software-based cell detection method to count single hematopoietic stem cells (HSCs) in microcavities. (3) Results: It was possible to detect single HSCs and their migration behavior within single microcavities. The HSCs displayed a pronounced migration behavior with one population of CD34-expressing cells located at the bottom of the microcavities and one population located in the middle of the microcavities at day 14. However, at day 21 the two populations seemed to unite again so that no clear distinction betwee ... mehr

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Verlagsausgabe §
DOI: 10.5445/IR/1000095723
Veröffentlicht am 11.06.2019
DOI: 10.3390/bioengineering6020050
Zugehörige Institution(en) am KIT Institut für Funktionelle Grenzflächen (IFG)
Publikationstyp Zeitschriftenaufsatz
Jahr 2019
Sprache Englisch
Identifikator ISSN: 2306-5354
KITopen-ID: 1000095723
HGF-Programm 47.02.06 (POF III, LK 01)
Erschienen in Bioengineering
Band 6
Heft 2
Seiten Article: 50
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Vorab online veröffentlicht am 31.05.2019
Schlagworte microcavity array; 3D cell culture; co-culture; hematopoietic progenitor cells; mesenchymal stromal cells; 4D microscopy platform
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