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Co-production of single cell oil and gluconic acid using oleaginous Cryptococcus podzolicus DSM 27192

Qian, X.; Gorte, O.; Chen, L.; Zhang, W.; Dong, W.; Ma, J.; Jiang, M.; Xin, F.; Ochsenreither, K.

Background: The co-production of single cell oil (SCO) with value-added products could improve the economic viability of industrial SCO production. The newly isolated oleaginous yeast Cryptococcus podzolicus DSM 27192 was able to co-produce SCO intracellularly and gluconic acid (GA) extracellularly. In this study, the metabolic regulation of carbon distribution between SCO and GA through process optimization was comprehensively investigated.
Results: The carbon flow distribution between SCO and GA was significantly influenced by the cultivation conditions, such as nitrogen sources, glucose concentration and dissolved oxygen concentration. It was found that organic nitrogen sources were beneficial for SCO accumulation, while GA production was decreased. Dissolved oxygen concentration (DOC) was found to enhance SCO accumulation, while high glucose concentration was more favorable for GA accumulation. Hence, a two-stage DOC or glucose concentration-controlled strategy was designed to improve cell growth and direct carbon distribution between SCO and GA. Moreover, C. podzolicus DSM 27192 could degrade its stored lipids to synthesize GA in the late stationary phase, although considerable amounts of glucose remained unconsumed in the culture medium, indicating the importance of fermentation time control in co-production systems. ... mehr

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Verlagsausgabe §
DOI: 10.5445/IR/1000096229
Veröffentlicht am 17.07.2019
DOI: 10.1186/s13068-019-1469-9
Zitationen: 5
Web of Science
Zitationen: 4
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Bio- und Lebensmitteltechnik (BLT)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2019
Sprache Englisch
Identifikator ISSN: 1754-6834
KITopen-ID: 1000096229
Erschienen in Biotechnology for biofuels
Band 12
Heft 1
Seiten Art. Nr.: 127
Vorab online veröffentlicht am 21.05.2019
Schlagwörter Co-production, Single cell oil (SCO), Gluconic acid (GA), Regulation, Carbon flow
Nachgewiesen in Scopus
Web of Science
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