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An Arabidopsis FANCJ helicase homologue is required for DNA crosslink repair and rDNA repeat stability

Dorn, A.; Feller, L.; Castri, D.; Röhrig, S.; Enderle, J.; Herrmann, N. J.; Block-Schmidt, A.; Trapp, O.; Köhler, L.; Puchta, H.

Proteins of the Fanconi Anemia (FA) complementation group are required for crosslink (CL) repair in humans and their loss leads to severe pathological phenotypes. Here we characterize a homolog of the Fe-S cluster helicase FANCJ in the model plant Arabidopsis, AtFANCJB, and show that it is involved in interstrand CL repair. It acts at a presumably early step in concert with the nuclease FAN1 but independently of the nuclease AtMUS81, and is epistatic to both error-prone and error-free post-replicative repair in Arabidopsis. The simultaneous knock out of FANCJB and the Fe-S cluster helicase RTEL1 leads to induced cell death in root meristems, indicating an important role of the enzymes in replicative DNA repair. Surprisingly, we found that AtFANCJB is involved in safeguarding rDNA stability in plants. In the absence of AtRTEL1 and AtFANCJB, we detected a synergetic reduction to about one third of the original number of 45S rDNA copies. It is tempting to speculate that the detected rDNA instability might be due to deficiencies in G-quadruplex structure resolution and might thus contribute to pathological phenotypes of certain human genetic diseases.

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Verlagsausgabe §
DOI: 10.5445/IR/1000096230
Veröffentlicht am 12.07.2019
DOI: 10.1371/journal.pgen.1008174
Zitationen: 3
Web of Science
Zitationen: 3
Cover der Publikation
Zugehörige Institution(en) am KIT Botanisches Institut (BOTANIK)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2019
Sprache Englisch
Identifikator ISSN: 1553-7390, 1553-7404
KITopen-ID: 1000096230
Erschienen in PLoS Genetics
Band 15
Heft 5
Seiten Art. Nr.: e1008174
Projektinformation CRISBREED (EU, H2020, 741306)
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Vorab online veröffentlicht am 23.05.2019
Nachgewiesen in Web of Science
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