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YB-1 recruitment to stress granules in zebrafish cells reveals a differential adaptive response to stress

Guarino, Andrea Maria; Mauro, Giuseppe Di; Ruggiero, Gennaro; Geyer, Nathalie; Delicato, Antonella; Foulkes, Nicholas S.; Vallone, Daniela; Calabrò, Viola

The survival of cells exposed to adverse environmental conditions entails various alterations in cellular function including major changes in the transcriptome as well as a radical reprogramming of protein translation. While in mammals this process has been extensively studied, stress responses in non-mammalian vertebrates remain poorly understood. One of the key cellular responses to many different types of stressors is the transient generation of structures called stress granules (SGs). These represent cytoplasmic foci where untranslated mRNAs are sorted or processed for re-initiation, degradation, or packaging into mRNPs. Here, using the evolutionarily conserved Y-box binding protein 1 (YB-1) and G3BP1 as markers, we have studied the formation of stress granules in zebrafish (D. rerio) in response to different environmental stressors. We show that following heat shock, zebrafish cells, like mammalian cells, form stress granules which contain both YB-1 and G3BP1 proteins. Moreover, zfYB-1 knockdown compromises cell viability, as well as recruitment of G3BP1 into SGs, under heat shock conditions highlighting the essential role played by YB-1 in SG assembly and cell survival. ... mehr

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Verlagsausgabe §
DOI: 10.5445/IR/1000096347
Veröffentlicht am 09.07.2019
DOI: 10.1038/s41598-019-45468-6
Zitationen: 1
Web of Science
Zitationen: 2
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Toxikologie und Genetik (ITG)
Publikationstyp Zeitschriftenaufsatz
Publikationsmonat/-jahr 12.2019
Sprache Englisch
Identifikator ISSN: 2045-2322
KITopen-ID: 1000096347
HGF-Programm 47.01.01 (POF III, LK 01)
Biol.Netzwerke u.Synth.Regulat. ITG+ITC
Erschienen in Scientific reports
Band 9
Heft 1
Seiten Article no: 9059
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Vorab online veröffentlicht am 21.06.2019
Nachgewiesen in Web of Science
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