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A Peptoid Delivers CoQ-derivative to Plant Mitochondria via Endocytosis

Asfaw, Kinfemichael Geressu 1; Liu, Qiong 1; Maisch, Jan 1; Münch, Stephan W. 2; Wehl, Ilona 3; Bräse, Stefan 2,3; Bogeski, Ivan; Schepers, Ute 2,3; Nick, Peter 1
1 Karlsruher Institut für Technologie (KIT)
2 Institut für Organische Chemie (IOC), Karlsruher Institut für Technologie (KIT)
3 Institut für Funktionelle Grenzflächen (IFG), Karlsruher Institut für Technologie (KIT)

Controlled delivery of molecules interfering specifically with target activities in a cell of interest can be a powerful tool for experimental manipulation, because it can be administered at a defined time point and does not require genetic transformation, which in some systems is difficult and time consuming. Peptides as versatile tools that can be tailored for binding numerous binding partners, are of special interest. However, their passage through membranes, their intracellular targeting, and their sensitivity to proteases is limiting. The use of peptoids, where cationic amino-acid side chains are linked to nitrogen (rather than to carbon) of the peptide bond, can circumvent these limitations, because they are not cleavable by proteases. In the current work, we provide a proof-of-concept that such Trojan Peptoids, the plant PeptoQ, can be used to target a functional cargo (i.e. a rhodamine-labelled peptoid and a coenzyme Q10 derivative) into mitochondria of tobacco BY-2 cells as experimental model. We show that the uptake is specific for mitochondria, rapid, dose-dependent, and requires clathrin-mediated endocytosis, as well as actin filaments, while microtubules seem to be dispensable. ... mehr

Verlagsausgabe §
DOI: 10.5445/IR/1000096481
Veröffentlicht am 22.07.2019
DOI: 10.1038/s41598-019-46182-z
Zitationen: 3
Zitationen: 4
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Funktionelle Grenzflächen (IFG)
Institut für Toxikologie und Genetik (ITG)
Publikationstyp Zeitschriftenaufsatz
Publikationsmonat/-jahr 12.2019
Sprache Englisch
Identifikator ISSN: 2045-2322
KITopen-ID: 1000096481
HGF-Programm 47.01.01 (POF III, LK 01) Biol.Netzwerke u.Synth.Regulat. ITG+ITC
Weitere HGF-Programme 47.02.06 (POF III, LK 01) Zellpopul.auf Biofunk.Oberflächen IFG
Erschienen in Scientific reports
Verlag Nature Research
Band 9
Heft 1
Seiten Art.Nr.: 9839
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Vorab online veröffentlicht am 08.07.2019
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