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Chemical characterization and in vitro toxicity on human bronchial epithelial cells BEAS-2B of PM$_{2.5}$ from an urban site under industrial emission influence

Courcot, Dominique; Landkocz, Yann; Billet, Sylvain; Ledoux, Frédéric; Verdin, Anthony; Martin, Perrine; Cazier, Fabrice

Particulate Matter (PM) is one of the most relevant environment-related health issues all over the world. In 2013, the International Agency for Research on Cancer (IARC) has classified air pollution and PM as a carcinogen for humans [1]. However, the mechanisms involved in the toxicity of these particles remains poorly understood, mainly because PM are uniquely complex owing to their physicochemical characteristics. In this study, fine particles were collected in the city center of Dunkirk, northern France using a 5 stages high volume cascade impactor (Staplex® 235, 68m3/h) and a Digitel DA80 high volume sampler (30m3/h).Samples were extensively characterized for their physico-chemical properties, including trace metals, water-soluble ions and organic species. Normal human bronchial epithelial cells (BEAS-2B) were used as cell model for toxicological analysis. Cytotoxicity, PAHs-metabolizing enzymes gene expression and genotoxic alterations were evaluated after 24, 48 or 72 h of exposure considering increasing concentrations of PM$_{2.5-0.3}$, organic extracts (OE) and water-soluble fraction (WF) of PM$_{2.5-0.3}$ and PM$_{2.5}$. Several sources such as road traffic, industrial activities mainly related to steelmaking, marine emissions including sea-salts and shipping, as well as soil resuspension were found to contribute to the PM$_{2.5}$ composition. ... mehr

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Volltext §
DOI: 10.5445/IR/1000096838
Veröffentlicht am 25.07.2019
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Zugehörige Institution(en) am KIT Institut für Meteorologie und Klimaforschung - Atmosphärische Aerosolforschung (IMK-AAF)
KIT-Zentrum Klima und Umwelt (ZKU)
Publikationstyp Vortrag
Publikationsdatum 16.02.2019
Sprache Englisch
Identifikator KITopen-ID: 1000096838
Veranstaltung 7th International Symposium on Ultrafine Particles, Air Quality and Climate (2019), Brüssel, Belgien, 15.05.2019 – 16.05.2019
Schlagwörter Fine and ultrafine particles, chemical composition, BEAS-2B cells, cytotoxicity, biotransformation, genotoxicity
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