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Application of diffractive optical elements in confocal microscopy

Li, Zheng

Abstract:
In traditional confocal microscopy, there is a tradeoff between the spatial resolution and the field of view due to the limitations of the objective lenses. To solve this problem, diffractive optical elements (DOEs) are used to generate illumination spots with high NA in a large area simultaneously. However, such DOEs in current research are only used as illuminators. In this work, the idea of superposition is utilized in DOEs to have flexible functionality and replace high-NA objectives for confocal measurement. To design the DOEs, different numerical simulation methods for light propagation are investigated and compared. Rayleigh-Sommerfeld integral is chosen to simulate the DOEs to get accurate results. Two kinds of DOEs are designed and simulated for 3D confocal surface measurements.

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Verlagsausgabe §
DOI: 10.5445/IR/1000097084
Veröffentlicht am 02.08.2019
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Anthropomatik (IFA)
Publikationstyp Proceedingsbeitrag
Publikationsjahr 2019
Sprache Englisch
Identifikator ISBN: 978-3-7315-0936-3
ISSN: 1863-6489
KITopen-ID: 1000097084
Erschienen in Proceedings of the 2018 Joint Workshop of Fraunhofer IOSB and Institute for Anthropomatics, Vision and Fusion Laboratory. Ed.: J. Beyerer, M. Taphanel
Verlag KIT Scientific Publishing
Seiten 25-46
Serie Karlsruher Schriften zur Anthropomatik / Lehrstuhl für Interaktive Echtzeitsysteme, Karlsruher Institut für Technologie ; Fraunhofer-Inst. für Optronik, Systemtechnik und Bildauswertung IOSB Karlsruhe ; 40
Bemerkung zur Veröffentlichung Technical Report IES-2018-03
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