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Ocean acidification has little effect on the biochemical composition of the coccolithophore Emiliania huxleyi

Heidenreich, Elena; Wördenweber, Robin; Kirschhöfer, Frank; Nusser, Michael; Friedrich, Frank; Fahl, Kirsten; Kruse, Olaf; Rost, Björn; Franzreb, Matthias; Brenner-Weiß, Gerald; Rokitta, Sebastian

Owing to the hierarchical organization of biology, from genomes over transcriptomes and proteomes down to metabolomes, there is continuous debate about the extent to which data and interpretations derived from one level, e.g. the transcriptome, are in agreement with other levels, e.g. the metabolome. Here, we tested the effect of ocean acidification (OA; 400 vs. 1000 μatm CO2) and its modulation by light intensity (50 vs. 300 μmol photons m-2 s-1) on the biomass composition (represented by 75 key metabolites) of diploid and haploid life-cycle stages of the coccolithophore Emiliania huxleyi (RCC1216 and RCC1217) and compared these data with interpretations from previous physiological and gene expression screenings. The metabolite patterns showed minor responses to OA in both life-cycle stages. Whereas previous gene expression analyses suggested that the observed increased biomass buildup derived from lipid and carbohydrate storage, this dataset suggests that OA slightly increases overall biomass of cells, but does not significantly alter their metabolite composition. Generally, light was shown to be a more dominant driver of metabolite composition than OA, increasing the relative abundances of amino acids, mannitol and storage lipids, and shifting pigment contents to accommodate increased irradiance levels. ... mehr

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Verlagsausgabe §
DOI: 10.5445/IR/1000097091
Veröffentlicht am 02.08.2019
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Funktionelle Grenzflächen (IFG)
Kompetenzzentrum für Materialfeuchte (CMM)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2019
Sprache Englisch
Identifikator ISSN: 1932-6203
KITopen-ID: 1000097091
HGF-Programm 47.02.06 (POF III, LK 01)
Zellpopul.auf Biofunk.Oberflächen IFG
Erschienen in PLOS ONE
Band 14
Heft 7
Seiten e0218564
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Vorab online veröffentlicht am 10.07.2019
Nachgewiesen in Scopus
Web of Science
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