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Delay in development and behavioural abnormalities in the absence of p53 in zebrafish

Elabd, Seham; Jabeen, Nuzhat Amna; Gerber, Vanessa; Peravali, Ravindra; Bourdon, J.-C.; Kancherla, Shilpa; Vallone, Daniela; Blattner, Christine

p53 is well-known for its tumour-suppressive activity. However, in the past decade it became clear that p53 is also involved in other processes including stem cell proliferation, differentiation and animal development. To investigate the role of p53 in early embryonic development, we targeted p53 by CRISPR/Cas9 to make a p53 knock-out zebrafish (Danio rerio). Our data show developmental and behavioural effects in p53-deficient zebrafish embryos and larvae. Specifically, we found that early development of zebrafish was clearly delayed in the absence of p53. However, after 1 day (1 dpf), the p53-deficient embryos appeared to recover, as evidenced by a similar level of pigmentation at 26 hpf, similar size of the eye at 4 dpf and only a minor difference in body size at 4 dpf compared to p53 wild-type siblings. The recovery of development after 1 dpf in p53-deficient embryos could be due to a compensatory mechanism involving other p53 family members. p63 and p73 were found over-expressed with respect to wild-type siblings. However, despite this adaptation, the hatching time remained delayed in p53$^{-/-}$ zebrafish. In addition to differences in development, p53-null zebrafish embryos also showed differences in behaviour. ... mehr

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Verlagsausgabe §
DOI: 10.5445/IR/1000097282
Veröffentlicht am 04.09.2019
DOI: 10.1371/journal.pone.0220069
Zitationen: 1
Web of Science
Zitationen: 1
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Toxikologie und Genetik (ITG)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2019
Sprache Englisch
Identifikator ISSN: 1932-6203
KITopen-ID: 1000097282
HGF-Programm 47.01.01 (POF III, LK 01)
Biol.Netzwerke u.Synth.Regulat. ITG+ITC
Erschienen in PLOS ONE
Band 14
Heft 7
Seiten Art.-Nr.: e0220069
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Vorab online veröffentlicht am 19.07.2019
Nachgewiesen in Scopus
Web of Science
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