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Turning a Killing Mechanism into an Adhesion and Antifouling Advantage

Dedisch, S.; Obstals, F.; Santos Pereira, A. de los; Bruns, Michael 1; Jakob, F.; Schwaneberg, U.; Rodriguez-Emmenegger, C.
1 Karlsruhe Nano Micro Facility (KNMF), Karlsruher Institut für Technologie (KIT)


Mild and universal methods to introduce functionality in polymeric surfaces remain a challenge. Herein, a bacterial killing mechanism based on amphiphilic antimicrobial peptides is turned into an adhesion advantage. Surface activity (surfactant) of the antimicrobial liquid chromatography peak I (LCI) peptide is exploited to achieve irreversible binding of a protein–polymer hybrid to surfaces via physical interactions. The protein–polymer hybrid consists of two blocks, a surface‐affine block (LCI) and a functional block to prevent protein fouling on surfaces by grafting antifouling polymers via single electron transfer‐living radical polymerization (SET‐LRP). The mild conditions of SET‐LRP of N‐2‐hydroxy propyl methacrylamide (HPMA) and carboxybetaine methacrylamide (CBMAA) preserve the secondary structure of the fusion protein. Adsorption kinetics and grafting densities are assessed using surface plasmon resonance and ellipsometry on model gold surfaces, while the functionalization of a range of artificial and natural surfaces, including teeth, is directly observed by confocal microscopy. Notably, the fusion protein modified with poly(HPMA) completely prevents the fouling from human blood plasma and thereby exhibits a resistance to protein fouling that is comparable to the best grafted‐from polymer brushes. ... mehr

Verlagsausgabe §
DOI: 10.5445/IR/1000097284
Veröffentlicht am 03.09.2019
DOI: 10.1002/admi.201900847
Zitationen: 16
Web of Science
Zitationen: 16
Zitationen: 18
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Angewandte Materialien – Energiespeichersysteme (IAM-ESS)
Karlsruhe Nano Micro Facility (KNMF)
Publikationstyp Zeitschriftenaufsatz
Publikationsdatum 20.09.2019
Sprache Englisch
Identifikator ISSN: 2196-7350
KITopen-ID: 1000097284
HGF-Programm 49.02.07 (POF III, LK 02) XPS
Erschienen in Advanced materials interfaces
Verlag John Wiley and Sons
Band 6
Heft 18
Seiten Art.-Nr.: 1900847
Vorab online veröffentlicht am 24.07.2019
Schlagwörter adhesion peptides, anchor peptides, antifouling, biomimetic coatings, SET-LRP
Nachgewiesen in Dimensions
Web of Science
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