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Automated high-throughput heart rate measurement in medaka and zebrafish embryos under physiological conditions

Gierten, J.; Pylatiuk, Christian; Hammouda, O.; Schock, Christian; Stegmaier, J.; Wittbrodt, J.; Gehrig, J.; Loosli, Felix

Rationale: Accurate and efficient quantification of heartbeats in small fish models is an important readout to study cardiovascular biology, disease states and pharmacology at large scale. However, dependence on anesthesia, laborious sample orientation or requirement for fluorescent reporters have hampered the establishment of high-throughput heartbeat analysis.

Objective: To overcome these limitations, we aimed to develop a high-throughput assay with automated heart rate scoring in medaka (Oryzias latipes) and zebrafish (Danio rerio) embryos under physiological conditions designed for genetic screens and drug discovery and validation.

Methods and Results: We established an efficient screening assay employing automated label-free heart rate determination of randomly oriented, non-anesthetized specimen in microtiter plates. Automatically acquired bright-field data feeds into an easy-to-use HeartBeat software, a MATLAB algorithm with graphical user interface developed for automated quantification of heart rate and rhythm. Sensitivity of the assay and algorithm was demonstrated by profiling heart rates during entire embryonic development. ... mehr

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Volltext §
DOI: 10.5445/IR/1000098307
Veröffentlicht am 17.09.2019
Zugehörige Institution(en) am KIT Institut für Toxikologie und Genetik (ITG)
Institut für Automation und angewandte Informatik (IAI)
Publikationstyp Forschungsbericht
Jahr 2019
Sprache Englisch
Identifikator KITopen-ID: 1000098307
HGF-Programm 47.01.02 (POF III, LK 01)
Umfang 39 S.
Schlagworte Zebrafish; medaka; automated microscopy; software-assisted heart rate quantification
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