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Pushing the mass limit for intact launch and photoionization of large neutral biopolymers

Schätti, J.; Rieser, P.; Sezer, U.; Richter, G.; Geyer, P.; Rondina, G. G.; Häussinger, D.; Mayor, Marcel; Shayeghi, A.; Köhler, V.; Arndt, M.

Abstract:
Since their first discovery by Louis Dunoyer and Otto Stern, molecular beams have conquered research and technology. However, it has remained an outstanding challenge to isolate and photoionize beams of massive neutral polypeptides. Here we show that femtosecond desorption from a matrix-free sample in high vacuum can produce biomolecular beams at least 25 times more efficiently than nanosecond techniques. While it has also been difficult to photoionize large biomolecules, we find that tailored structures with an abundant exposure of tryptophan residues at their surface can be ionized by vacuum ultraviolet light. The combination of these desorption and ionization techniques allows us to observe molecular beams of neutral polypeptides with a mass exceeding 20,000 amu. They are composed of 50 amino acids – 25 tryptophan and 25 lysine residues – and 26 fluorinated alkyl chains. The tools presented here offer a basis for the preparation, control and detection of polypeptide beams.

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Verlagsausgabe §
DOI: 10.5445/IR/1000098428
Veröffentlicht am 02.10.2019
Originalveröffentlichung
DOI: 10.1038/s42004-018-0095-y
Coverbild
Zugehörige Institution(en) am KIT Institut für Nanotechnologie (INT)
Publikationstyp Zeitschriftenaufsatz
Jahr 2018
Sprache Englisch
Identifikator ISSN: 2399-3669
KITopen-ID: 1000098428
HGF-Programm 43.21.04 (POF III, LK 01)
Erschienen in Communications chemistry
Band 1
Heft 1
Seiten 93
Nachgewiesen in Scopus
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