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Preparative Protein Crystallization

Hubbuch, J. ORCID iD icon 1; Kind, M. 1; Nirschl, H. 1
1 Karlsruher Institut für Technologie (KIT)

Abstract (englisch):

Preparative protein crystallization can possibly replace one or more chromatographic steps in downstream processing. The development of such crystallization processes is demanding: first, promising principal crystallization conditions must be identified; second, details about the process must be defined; and third, the crystals have to be separated from the mother liquor without putting harm of their crystalline integrity. State‐of‐the‐art about these three steps is developing fast by (i) employing new screening methods which are based on fundamental understanding of the interaction of the protein molecules, (ii) application of existing concepts of technical bulk crystallization of small molecules to preparative protein crystallization, and (iii) making available specific gentle separation machinery.

DOI: 10.1002/ceat.201800627
Zitationen: 12
Web of Science
Zitationen: 11
Zitationen: 13
Zugehörige Institution(en) am KIT Institut für Bio- und Lebensmitteltechnik (BLT)
Institut für Mechanische Verfahrenstechnik und Mechanik (MVM)
Institut für Thermische Verfahrenstechnik (TVT)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2019
Sprache Englisch
Identifikator ISSN: 0930-7516, 1521-4125
KITopen-ID: 1000098562
Erschienen in Chemical engineering & technology
Verlag John Wiley and Sons
Band 42
Heft 11
Seiten 2275-2281
Vorab online veröffentlicht am 12.08.2019
Nachgewiesen in Scopus
Web of Science
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