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The genome of Alcaligenes aquatilis strain BU33N: Insights into hydrocarbon degradation capacity

Mahjoubi, Mouna; Aliyu, Habibu 1; Cappello, Simone; Naifer, Mohamed; Souissi, Yasmine; Cowan, Don A.; Cherif, Ameur
1 Institut für Bio- und Lebensmitteltechnik (BLT), Karlsruher Institut für Technologie (KIT)


Environmental contamination with hydrocarbons though natural and anthropogenic activities is a serious threat to biodiversity and human health. Microbial bioremediation is considered as the effective means of treating such contamination. This study describes a biosurfactant producing bacterium capable of utilizing crude oil and various hydrocarbons as the sole carbon source. Strain BU33N was isolated from hydrocarbon polluted sediments from the Bizerte coast (northern Tunisia) and was identified as Alcaligenes aquatilis on the basis of 16S rRNA gene sequence analysis. When grown on crude oil and phenanthrene as sole carbon and energy sources, isolate BU33N was able to degrade ~86%, ~56% and 70% of TERHc, n-alkanes and phenanthrene, respectively. The draft genome sequence of the A. aquatilis strain BU33N was assembled into one scaffold of 3,838,299 bp (G+C content of 56.1%). Annotation of the BU33N genome resulted in 3,506 protein-coding genes and 56 rRNA genes. A large repertoire of genes related to the metabolism of aromatic compounds including genes encoding enzymes involved in the complete degradation of benzoate were identified. Also genes associated with resistance to heavy metals such as copper tolerance and cobalt-zinc-cadmium resistance were identified in BU33N. ... mehr

Verlagsausgabe §
DOI: 10.5445/IR/1000098622
Veröffentlicht am 01.10.2019
DOI: 10.1371/journal.pone.0221574
Zitationen: 19
Zitationen: 19
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Bio- und Lebensmitteltechnik (BLT)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2019
Sprache Englisch
Identifikator ISSN: 1932-6203
KITopen-ID: 1000098622
Erschienen in PLOS ONE
Verlag Public Library of Science (PLoS)
Band 14
Heft 9
Seiten Art. Nr.: e0221574
Vorab online veröffentlicht am 24.09.2019
Nachgewiesen in Scopus
Web of Science
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