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CRISPR/Cas brings plant biology and breeding into the fast lane

Schindele, Angelina; Dorn, Annika; Puchta, Holger

Abstract:
CRISPR/Cas is in the process of inducing the biggest transformation of plant breeding since the green revolution. Whereas initial efforts focused mainly on changing single traits by error prone non-homologous end joining, the last two years saw a tremendous technical progress achieving more complex genetic, epigenetic and transcriptional changes. The efficiencies of inducing directed changes by homologous recombination have been improved significantly and strategies to break genetic linkages by inducing chromosomal rearrangements have been developed. Cas13 systems have been applied to degrade viral and mRNA in plants. Most importantly, a historical breakthrough was accomplished: By introducing multiple genomic changes simultaneously, domestication of wild species in a single generation has been demonstrated, speeding up breeding dramatically.

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Postprint §
DOI: 10.5445/IR/1000098747
Veröffentlicht am 24.04.2020
Originalveröffentlichung
DOI: 10.1016/j.copbio.2019.08.006
Scopus
Zitationen: 8
Web of Science
Zitationen: 6
Cover der Publikation
Zugehörige Institution(en) am KIT Botanisches Institut (BOTANIK)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2020
Sprache Englisch
Identifikator ISSN: 0958-1669, 1879-0429
KITopen-ID: 1000098747
Erschienen in Current opinion in biotechnology
Band 61
Seiten 7-14
Projektinformation CRISBREED (EU, H2020, 741306)
Vorab online veröffentlicht am 23.09.2019
Nachgewiesen in Scopus
Web of Science
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