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Potential of biotechnological conversion of lignocellulose hydrolyzates by Pseudomonas putida KT2440 as a model organism for a bio‐based economy

Horlamus, Felix; Wang, Yan; Steinbach, David 1; Vahidinasab, Maliheh; Wittgens, Andreas; Rosenau, Frank; Henkel, Marius; Hausmann, Rudolf
1 Institut für Katalyseforschung und -technologie (IKFT), Karlsruher Institut für Technologie (KIT)

Abstract (englisch):

Lignocellulose‐derived hydrolyzates typically display a high degree of variation depending on applied biomass source material as well as process conditions. Consequently, this typically results in variable composition such as different sugar concentrations as well as degree and the presence of inhibitors formed during hydrolysis. These key obstacles commonly limit its efficient use as a carbon source for biotechnological conversion. The gram‐negative soil bacterium Pseudomonas putida KT2440 is a promising candidate for a future lignocellulose‐based biotechnology process due to its robustness and versatile metabolism. Recently, P. putida KT2440_xylAB which was able to metabolize the hemicellulose (HC) sugars, xylose and arabinose, was developed and characterized. Building on this, the intent of the study was to evaluate different lignocellulose hydrolyzates as platform substrates for P. putida KT2440 as a model organism for a bio‐based economy. Firstly, hydrolyzates of different origins were evaluated as potential carbon sources by cultivation experiments and determination of cell growth and sugar consumption. Secondly, the content of major toxic substances in cellulose and HC hydrolyzates was determined and their inhibitory effect on bacterial growth was characterized. ... mehr

Verlagsausgabe §
DOI: 10.5445/IR/1000098821
Veröffentlicht am 29.11.2019
DOI: 10.1111/gcbb.12647
Zitationen: 16
Zitationen: 17
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Katalyseforschung und -technologie (IKFT)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2019
Sprache Englisch
Identifikator ISSN: 1757-1693, 1757-1707
KITopen-ID: 1000098821
HGF-Programm 35.13.02 (POF III, LK 01) Konversion
Erschienen in Global change biology / Bioenergy
Verlag Wiley Open Access
Band 11
Heft 12
Seiten 1421-1434
Vorab online veröffentlicht am 06.10.2019
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Web of Science
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