KIT | KIT-Bibliothek | Impressum | Datenschutz

Potential of biotechnological conversion of lignocellulose hydrolyzates by Pseudomonas putida KT2440 as a model organism for a bio‐based economy [in press]

Horlamus, Felix; Wang, Yan; Steinbach, David; Vahidinasab, Maliheh; Wittgens, Andreas; Rosenau, Frank; Henkel, Marius; Hausmann, Rudolf

Abstract (englisch):
Lignocellulose‐derived hydrolyzates typically display a high degree of variation depending on applied biomass source material as well as process conditions. Consequently, this typically results in variable composition such as different sugar concentrations as well as degree and the presence of inhibitors formed during hydrolysis. These key obstacles commonly limit its efficient use as a carbon source for biotechnological conversion. The gram‐negative soil bacterium Pseudomonas putida KT2440 is a promising candidate for a future lignocellulose‐based biotechnology process due to its robustness and versatile metabolism. Recently, P. putida KT2440_xylAB which was able to metabolize the hemicellulose (HC) sugars, xylose and arabinose, was developed and characterized. Building on this, the intent of the study was to evaluate different lignocellulose hydrolyzates as platform substrates for P. putida KT2440 as a model organism for a bio‐based economy. Firstly, hydrolyzates of different origins were evaluated as potential carbon sources by cultivation experiments and determination of cell growth and sugar consumption. Secondly, the content of major toxic substances in cellulose and HC hydrolyzates was determined and their inhibitory effect on bacterial growth was characterized. ... mehr

Open Access Logo


Download
Originalveröffentlichung
DOI: 10.1111/gcbb.12647
Zugehörige Institution(en) am KIT Institut für Katalyseforschung und -Technologie (IKFT)
Publikationstyp Zeitschriftenaufsatz
Jahr 2019
Sprache Englisch
Identifikator ISSN: 1757-1693, 1757-1707
KITopen-ID: 1000098821
HGF-Programm 35.13.02 (POF III, LK 01)
Erschienen in Global change biology / Bioenergy
Seiten gcbb.12647
Vorab online veröffentlicht am 06.10.2019
KIT – Die Forschungsuniversität in der Helmholtz-Gemeinschaft
KITopen Landing Page