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BioSAXS Measurements Reveal That Two Antimicrobial Peptides Induce Similar Molecular Changes in Gram-Negative and Gram-Positive Bacteria

von Gundlach, Andreas; Ashby, Martin P.; Gani, Jurnorain; Lopez-Perez, Paula Matilde; Cookson, Alan Roy; Ann Huws, Sharon; Rumancev, Christoph; Garamus, Vasil M.; Mikut, Ralf; Rosenhahn, Axel; Hilpert, Kai

Abstract:
Two highly active short broad-spectrum AMPs (14D and 69D) with unknown mode of action have been investigated in regards to their effect against the Gramnegative bacteria Escherichia coli and the Gram-positive bacteria methicillinresistant Staphylococcus aureus (MRSA). Minimal inhibitory concentration (MIC) measurements using a cell density of 108 cfu/ml resulted in values between 16 and 32 μg/ml. Time-kill experiments using 108 cfu/ml revealed complete killing, except for 69D in combination with MRSA, where bacterial load was reduced a million times. Small-angle X-ray scattering of biological samples (BioSAXS) at 108 cfu/ml was applied to investigate the ultrastructural changes in E. coli and MRSA in response to these two broad-spectrum AMPs. In addition, electron microscopy (EM) was performed to visualize the treated and non-treated bacteria. As expected, the scattering curves generated using BioSAXS show the ultrastructure of the Grampositive and Gram-negative bacteria to be very different (BioSAXS is not susceptible to the outer shape). After treatment with either peptide, the scattering curves of E.coli and MRSA cells are much more alike. ... mehr

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Verlagsausgabe §
DOI: 10.5445/IR/1000098830
Veröffentlicht am 09.10.2019
Originalveröffentlichung
DOI: 10.3389/fphar.2019.01127
Coverbild
Zugehörige Institution(en) am KIT Institut für Automation und angewandte Informatik (IAI)
Publikationstyp Zeitschriftenaufsatz
Jahr 2019
Sprache Englisch
Identifikator ISSN: 1663-9812
KITopen-ID: 1000098830
HGF-Programm 47.01.02 (POF III, LK 01)
Erschienen in Frontiers in pharmacology
Band 10
Seiten Art. Nr.: 1127
Vorab online veröffentlicht am 26.09.2019
Schlagworte antimicrobial peptide, mode of action, SAXS, MRSA, electron microscopy, E. coli
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