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Histone H3K27 acetylation precedes active transcription during zebrafish zygotic genome activation as revealed by live-cell analysis

Sato, Yuko; Hilbert, Lennart; Oda, Haruka; Wan, Yinan; Heddleston, John M.; Chew, Teng-Leong; Zaburdaev, Vasily; Keller, Philipp; Lionnet, Timothee; Vastenhouw, Nadine; Kimura, Hiroshi

Abstract:
Histone post-translational modifications are key gene expression regulators, but their rapid dynamics during development remain difficult to capture. We applied a Fab-based live endogenous modification labeling technique to monitor the changes in histone modification levels during zygotic genome activation (ZGA) in living zebrafish embryos. Among various histone modifications, H3 Lys27 acetylation (H3K27ac) exhibited most drastic changes, accumulating in two nuclear foci in the 64- to 1k-cell-stage embryos. The elongating form of RNA polymerase II, which is phosphorylated at Ser2 in heptad repeats within the C-terminal domain (RNAP2 Ser2ph), and miR-430 transcripts were also concentrated in foci closely associated with H3K27ac. When treated with α-amanitin to inhibit transcription or JQ-1 to inhibit binding of acetyl-reader proteins, H3K27ac foci still appeared but RNAP2 Ser2ph and miR-430 morpholino were not concentrated in foci, suggesting that H3K27ac precedes active transcription during ZGA. We anticipate that the method presented here could be applied to a variety of developmental processes in any model and non-model organisms.

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Verlagsausgabe §
DOI: 10.5445/IR/1000099309
Veröffentlicht am 25.10.2019
Originalveröffentlichung
DOI: 10.1242/dev.179127
Scopus
Zitationen: 3
Web of Science
Zitationen: 2
Cover der Publikation
Zugehörige Institution(en) am KIT Zoologisches Institut (ZOO)
Institut für Toxikologie und Genetik (ITG)
Publikationstyp Zeitschriftenaufsatz
Publikationsmonat/-jahr 10.2019
Sprache Englisch
Identifikator ISSN: 0950-1991, 1477-9129
KITopen-ID: 1000099309
HGF-Programm 47.01.01 (POF III, LK 01)
Biol.Netzwerke u.Synth.Regulat. ITG+ITC
Erschienen in Development <Cambridge>
Band 146
Heft 19
Seiten Article: dev179127
Vorab online veröffentlicht am 30.09.2019
Nachgewiesen in Web of Science
Scopus
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