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Screening and comparative characterization of microorganisms from Iranian soil samples showing ω-transaminase activity toward a plethora of substrates

Noshahri, N. G.; Fooladi, J.; Syldatk, C.; Engel, U.; Heravi, M. M.; Mehrjerdi, M. Z.; Rudat, J.

Abstract (englisch):
In this study, soil microorganisms from Iran were screened for ω-transaminase (ω-TA) activity based on growth on minimal media containing (rac)-α-methylbenzylamine (rac-α-MBA) as a sole nitrogen source. Then, for the selection of strains with high enzyme activity, a colorimetric o-xylylendiamine assay was conducted. The most promising strains were identified by 16S rDNA sequencing. Five microorganisms showing high ω-TA activity were subjected to determine optimal conditions for ω-TA activity, including pH, temperature, co-solvent, and the specificity of the ω-TA toward different amine donors and acceptors. Among the five screened microorganisms, Bacillus halotolerans turned out to be the most promising strain: Its cell-free extract showed a highly versatile amino donor spectrum toward aliphatic, aromatic chiral amines and a broad range of pH activity. Transaminase activity also exhibited excellent solvent tolerance, with maximum turnover in the presence of 30% (v/v) DMSO

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Verlagsausgabe §
DOI: 10.5445/IR/1000099771
Veröffentlicht am 13.11.2019
DOI: 10.3390/catal9100874
Zitationen: 1
Web of Science
Zitationen: 1
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Bio- und Lebensmitteltechnik (BLT)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2019
Sprache Englisch
Identifikator ISSN: 2073-4344
KITopen-ID: 1000099771
Erschienen in Catalysts
Band 9
Heft 10
Seiten Article No.874
Vorab online veröffentlicht am 22.10.2019
Schlagwörter ω-transaminase; α-methylbenzylamine; biocatalysis; chiral amine; biotransformation
Nachgewiesen in Web of Science
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