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Valency engineering of monomeric enzymes for self-assembling biocatalytic hydrogels

Bitterwolf, Patrick; Gallus, Sabrina; Peschke, Theo; Mittmann, Esther; Oelschlaeger, Claude; Willenbacher, Norbert; Rabe, Kersten S.; Niemeyer, Christof M.

All-enzyme hydrogels are efficient reagents for continuous flow biocatalysis. These materials can be obtained by self-assembly of two oligomeric enzymes, modified with the complementary SpyTag and SpyCatcher units. To facilitate access to the large proportion of biocatalytically relevant monomeric enzymes, we demonstrate that the tagging valency of the monomeric (S)-stereoselective ketoreductase Gre2p from Saccharomyces cerevisiae can be designed to assemble stable, active hydrogels with the cofactor-regenerating glucose 1-dehydrogenase GDH from Bacillus subtilis. Mounted in microfluidic reactors, these gels revealed high conversion rates and stereoselectivity in the reduction of prochiral methylketones under continuous flow for more than 8 days. The sequential use as well as parallelization by ‘numbering up’ of the flow reactor modules demonstrate that this approach is suitable for syntheses on the semipreparative scale.

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Verlagsausgabe §
DOI: 10.5445/IR/1000099957
Veröffentlicht am 18.11.2019
DOI: 10.1039/c9sc04074a
Zitationen: 11
Web of Science
Zitationen: 11
Zitationen: 11
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Biologische Grenzflächen (IBG)
Institut für Mechanische Verfahrenstechnik und Mechanik (MVM)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2019
Sprache Englisch
Identifikator ISSN: 2041-6520, 2041-6539
KITopen-ID: 1000099957
HGF-Programm 47.02.01 (POF III, LK 01) Zellpopul.auf Biofunk.Oberflächen IBG-1
Erschienen in Chemical science
Verlag Royal Society of Chemistry (RSC)
Band 10
Heft 42
Seiten 9752–9757
Vorab online veröffentlicht am 06.09.2019
Nachgewiesen in Web of Science
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