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Water on hydrophobic surfaces: mechanistic modeling of polyethylene glycol-induced protein precipitation

Großhans, Steffen 1; Wang, Gang 1; Hubbuch, Jürgen ORCID iD icon 1
1 Institut für Bio- und Lebensmitteltechnik (BLT), Karlsruher Institut für Technologie (KIT)


For the purification of biopharmaceutical proteins, liquid chromatography is still the gold standard. Especially with increasing product titers, drawbacks like slow volumetric throughput and high resin costs lead to an intensifying need for alternative technologies. Selective preparative protein precipitation is one promising alternative technique. Although the capability has been proven, there has been no precipitation process realized for large-scale monoclonal antibody (mAb) production yet. One reason might be that the mechanism behind protein phase behavior is not completely understood and the precipitation process development is still empirical. Mechanistic modeling can be a means for faster, material-saving process development and a better process understanding at the same time. In preparative chromatography, mechanistic modeling was successfully shown for a variety of applications. Lately, a new isotherm for hydrophobic interaction chromatography (HIC) under consideration of water molecules as participants was proposed, enabling an accurate description of HIC. In this work, based on similarities between protein precipitation and HIC, a new precipitation model was derived. ... mehr

Verlagsausgabe §
DOI: 10.5445/IR/1000100240
Veröffentlicht am 25.11.2019
DOI: 10.1007/s00449-018-2054-5
Zitationen: 7
Web of Science
Zitationen: 6
Zitationen: 8
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Bio- und Lebensmitteltechnik (BLT)
Publikationstyp Zeitschriftenaufsatz
Publikationsmonat/-jahr 04.2019
Sprache Englisch
Identifikator ISSN: 1615-7591, 1615-7605
KITopen-ID: 1000100240
Erschienen in Bioprocess and biosystems engineering
Verlag Springer
Band 42
Heft 4
Seiten 513–520
Projektinformation nextBioPharmDSP (EU, H2020, 635557)
Vorab online veröffentlicht am 07.12.2018
Schlagwörter Preparative protein precipitation, Polyethylene glycol, Mechanistic modeling, Water structure, Industrial monoclonal antibody
Nachgewiesen in Web of Science
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