KIT | KIT-Bibliothek | Impressum | Datenschutz

Inductively coupled magic angle spinning microresonators benchmarked for high-resolution single embryo metabolomic profiling

Adhikari, Shyam S.; Zhao, Li; Dickmeis, Thomas; Korvink, Jan G.; Badilita, Vlad

Abstract:
The magic angle coil spinning (MACS) technique has been introduced as a very promising extension for solid state NMR detection, demonstrating sensitivity enhancements by a factor of 14 from the very first time it has been reported. The main beneficiary of this technique is the scientific community dealing with mass- and volume-limited, rare, or expensive samples. However, more than a decade after the first report on MACS, there is a very limited number of groups who have continued to develop the technique, let alone it being widely adopted by practitioners. This might be due to several drawbacks associated with the MACS technology until now, including spectral linewidth, heating due to eddy currents, and imprecise manufacturing. Here, we report a device overcoming all these remaining issues, therefore achieving: (1) spectral resolution of approx 0.01 ppm and normalized limit of detection of approx. 13 nmol s⁰·⁵calculated using the anomeric proton of sucrose at 3 kHz MAS frequency; (2) limited temperature increase inside the MACS insert of only 5 °C at 5 kHz MAS frequency in an 11.74 T magnetic field, rendering MACS suitable to study live biological samples. ... mehr

Open Access Logo


Verlagsausgabe §
DOI: 10.5445/IR/1000100297
Veröffentlicht am 06.12.2019
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Mikrostrukturtechnik (IMT)
Institut für Toxikologie und Genetik (ITG)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2019
Sprache Englisch
Identifikator ISSN: 0003-2654, 1364-5528
KITopen-ID: 1000100297
HGF-Programm 43.22.01 (POF III, LK 01)
Functionality by Design
Erschienen in The analyst
Band 144
Heft 24
Seiten 7192-7199
Vorab online veröffentlicht am 29.10.2019
Nachgewiesen in Web of Science
Scopus
KIT – Die Forschungsuniversität in der Helmholtz-Gemeinschaft
KITopen Landing Page