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Synergy Pattern of Short Cationic Antimicrobial Peptides Against Multidrug-Resistant Pseudomonas aeruginosa

Ruden, Serge; Rieder, Annika; Chis Ster, Irina; Schwartz, Thomas; Mikut, Ralf; Hilpert, Kai

Abstract:
With the rise of various multidrug-resistant (MDR) pathogenic bacteria, worldwide health care is under pressure to respond. Conventional antibiotics are failing and the development of novel classes and alternative strategies is a major priority. Antimicrobial peptides (AMPs) cannot only kill MDR bacteria, but also can be used synergistically with conventional antibiotics. We selected 30 short AMPs from different origins and measured their synergy in combination with polymyxin B, piperacillin, ceftazidime, cefepime, meropenem, imipenem, tetracycline, erythromycin, kanamycin, tobramycin, amikacin, gentamycin, and ciprofloxacin. In total, 403 unique combinations were tested against an MDR Pseudomonas aeruginosa isolate (PA910). As a measure of the synergistic effects, fractional inhibitory concentrations (FICs) were determined using microdilution assays with FICs ranges between 0.25 and 2. A high number of combinations between peptides and polymyxin B, erythromycin, and tetracycline were found to be synergistic. Novel variants of indolicidin also showed a high frequency in synergist interaction. Single amino acid substitutions within the peptides can have a very strong effect on the ability to synergize, making it possible to optimize future drugs toward synergistic interaction.

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Verlagsausgabe §
DOI: 10.5445/IR/1000100488
Veröffentlicht am 16.01.2020
Coverbild
Zugehörige Institution(en) am KIT Institut für Mikrostrukturtechnik (IMT)
Institut für Funktionelle Grenzflächen (IFG)
Institut für Biologische Grenzflächen (IBG)
Institut für Automation und angewandte Informatik (IAI)
Publikationstyp Zeitschriftenaufsatz
Jahr 2019
Sprache Englisch
Identifikator ISSN: 1664-302X
KITopen-ID: 1000100488
HGF-Programm 47.01.02 (POF III, LK 01)
Erschienen in Frontiers in microbiology
Band 10
Seiten Art. Nr.: 2740
Vorab online veröffentlicht am 28.11.2019
Schlagworte multidrug resistance, Pseudomonas aeruginosa, antimicrobial peptides, synergy, revive old antibiotics
Nachgewiesen in Scopus
Web of Science
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