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Imine Reductase Based All-Enzyme Hydrogel with Intrinsic Cofactor Regeneration for Flow Biocatalysis

Bitterwolf, Patrick; Ott, Felix; Rabe, Kersten S.; Niemeyer, Christof M.

Abstract (englisch):
All-enzyme hydrogels are biocatalytic materials, with which various enzymes can be immobilized in microreactors in a simple, mild, and efficient manner to be used for continuous flow processes. Here we present the construction and application of a cofactor regenerating hydrogel based on the imine reductase GF3546 from Streptomyces sp. combined with the cofactor regenerating glucose-1-dehydrogenase from Bacillus subtilis. The resulting hydrogel materials were characterized in terms of binding kinetics and viscoelastic properties. The materials were formed by rapid covalent crosslinking in less than 5 min, and they showed a typical mesh size of 67 ± 2 nm. The gels were applied for continuous flow biocatalysis. In a microfluidic reactor setup, the hydrogels showed excellent conversions of imines to amines for up to 40 h in continuous flow mode. Variation of flow rates led to a process where the gels showed a maximum space-time-yield of 150 g·(L·day)−1 at 100 μL/min

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Verlagsausgabe §
DOI: 10.5445/IR/1000100673
Veröffentlicht am 09.12.2019
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Biologische Grenzflächen (IBG)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2019
Sprache Englisch
Identifikator ISSN: 2072-666X
KITopen-ID: 1000100673
HGF-Programm 47.02.01 (POF III, LK 01)
Zellpopul.auf Biofunk.Oberflächen IBG-1
Erschienen in Micromachines
Band 10
Heft 11
Seiten Article No.783
Vorab online veröffentlicht am 15.11.2019
Schlagwörter enzyme immobilization; continuous; biocatalysis; imine reductase; microreactor
Nachgewiesen in Scopus
Web of Science
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