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Scalable dissolution-dynamic nuclear polarization with rapid transfer of a polarized solid

Kouřil, Karel; Kouřilová, Hana; Bartram, Samuel; Levitt, Malcolm H.; Meier, Benno

Abstract (englisch):
In dissolution-dynamic nuclear polarization, nuclear spins are hyperpolarized at cryogenic temperatures using radicals and microwave irradiation. The hyperpolarized solid is dissolved with hot solvent and the solution is transferred to a secondary magnet where strongly enhanced magnetic resonance signals are observed. Here we present a method for transferring the hyperpolarized solid. A bullet containing the frozen, hyperpolarized sample is ejected using pressurized helium gas, and shot into a receiving structure in the secondary magnet, where the bullet is retained and the polarized solid is dissolved rapidly. The transfer takes approximately 70 ms. A solenoid, wound along the entire transfer path ensures adiabatic transfer and limits radical-induced low-field relaxation. The method is fast and scalable towards small volumes suitable for high-resolution nuclear magnetic resonance spectroscopy while maintaining high concentrations of the target molecule. Polarization levels of approximately 30% have been observed for 1-13C-labelled pyruvic acid in solution.

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Verlagsausgabe §
DOI: 10.5445/IR/1000100825
Veröffentlicht am 12.12.2019
Originalveröffentlichung
DOI: 10.1038/s41467-019-09726-5
Scopus
Zitationen: 6
Web of Science
Zitationen: 5
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Biologische Grenzflächen (IBG)
Publikationstyp Zeitschriftenaufsatz
Publikationsmonat/-jahr 12.2019
Sprache Englisch
Identifikator ISSN: 2041-1723
KITopen-ID: 1000100825
HGF-Programm 47.02.04 (POF III, LK 01)
Zellpopul.auf Biofunk.Oberflächen IBG-4
Erschienen in Nature Communications
Band 10
Heft 1
Seiten Article No.1733
Vorab online veröffentlicht am 15.04.2019
Nachgewiesen in Scopus
Web of Science
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