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A single Thaumarchaeon drives nitrification in deep oligotrophic Lake Constance

Herber, J.; Klotz, F.; Frommeyer, B.; Weis, S.; Straile, D.; Kolar, Allison 1,2; Sikorski, J.; Egert, M.; Dannenmann, M. 1,2; Pester, M.
1 Institut für Meteorologie und Klimaforschung (IMK), Karlsruher Institut für Technologie (KIT)
2 Institut für Meteorologie und Klimaforschung – Atmosphärische Umweltforschung (IMK-IFU), Karlsruher Institut für Technologie (KIT)

Ammonia released during organic matter mineralization is converted during nitrification to nitrate. We followed spatiotemporal dynamics of the nitrifying microbial community in deep oligotrophic Lake Constance. Depth‐dependent decrease of total ammonium (0.01–0.84 μM) indicated the hypolimnion as the major place of nitrification with $^{15}$N‐isotope dilution measurements indicating a threefold daily turnover of hypolimnetic total ammonium. This was mirrored by a strong increase of ammonia‐oxidizing Thaumarchaeota towards the hypolimnion (13%–21% of bacterioplankton) throughout spring to autumn as revealed by amplicon sequencing and quantitative polymerase chain reaction. Ammonia‐oxidizing bacteria were typically two orders of magnitude less abundant and completely ammonia‐oxidizing (comammox) bacteria were not detected. Both, 16S rRNA gene and amoA (encoding ammonia monooxygenase subunit B) analyses identified only one major species‐level operational taxonomic unit (OTU) of Thaumarchaeota (99% of all ammonia oxidizers in the hypolimnion), which was affiliated to Nitrosopumilus spp. The relative abundance distribution of the single Thaumarchaeon strongly correlated to an equally abundant Chloroflexi clade CL500‐11 OTU and a Nitrospira OTU that was one order of magnitude less abundant. ... mehr

Verlagsausgabe §
DOI: 10.5445/IR/1000104257
Veröffentlicht am 16.12.2019
DOI: 10.1111/1462-2920.14840
Zitationen: 14
Web of Science
Zitationen: 14
Zitationen: 18
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Meteorologie und Klimaforschung – Atmosphärische Umweltforschung (IMK-IFU)
KIT-Zentrum Klima und Umwelt (ZKU)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2020
Sprache Englisch
Identifikator ISSN: 1462-2912, 1462-2920
KITopen-ID: 1000104257
HGF-Programm 12.02.01 (POF III, LK 01) Effects of land use and climate change
Erschienen in Environmental microbiology
Verlag John Wiley and Sons
Band 22
Heft 1
Seiten 212-228
Nachgewiesen in Dimensions
Web of Science
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