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Targeted UPLC/MS/MS quantification of methylated amines and selected amino acids in biofluids

Roggensack, Tim; Merz, Benedikt; Dick, Niels; Bub, Achim; Krüger, Ralf

Methylated amino compounds and basic amino acids are important analyte classes with high relevance in nutrition, physical activity and physiology. Reliable and easy quantification methods covering a variety of metabolites in body fluids are a prerequisite for efficient investigations in the field of food and nutrition.
Targeted UHPLC/MS analysis was performed using HILIC separation and timed ESI-MRM detection, combined with a short sample preparation. Calibration in urine and blood plasma was achieved by matrix-matched standards, isotope-labelled internal standards and standard addition. The method was fully validated and the performance was evaluated using a subset from the Karlsruhe Metabolomics and Nutrition (KarMeN) study.
Within this method, a number of 30 compounds could be quantified simultaneously in a short run of 9 minutes in both body fluids. It covers a variety of free amino compounds which are present in very different concentrations. The method is easy, precise and robust, and has a broad working range. As a proof of principle, literature-based associations of certain metabolites with dietary intake of respective foods were clearly confirmed in the KarMeN subset.
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Verlagsausgabe §
DOI: 10.5445/IR/1000105194
Veröffentlicht am 24.09.2020
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Sport und Sportwissenschaft (IfSS)
Publikationstyp Zeitschriftenaufsatz
Publikationsdatum 15.04.2020
Sprache Englisch
Identifikator ISSN: 0951-4198, 1097-0231
KITopen-ID: 1000105194
Erschienen in Rapid communications in mass spectrometry
Band 34
Heft 7
Seiten Art.Nr. e8646
Vorab online veröffentlicht am 31.10.2019
Schlagwörter methylated amine, amino acid, HILIC/MS, nutrition, dietary intake marker
Nachgewiesen in Scopus
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