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Biolasing from Individual Cells in a Low‐Q Resonator Enables Spectral Fingerprinting

Septiadi, Dedy; Barna, Valentin; Saxena, Dhruv; Sapienza, Riccardo; Genovese, Damiano; De Cola, Luisa

Abstract:
Lasing from cells has recently been subject of thorough investigation because of the potential for sensitive and fast biosensing. Yet, lasing from individual cells has been studied in high‐quality resonators, resulting in limited dependence of the lasing properties on the cellular microenvironment. Here, lasing is triggered by cells floating in a low quality factor resonator composed of a disposable poly(methyl methacrylate) (PMMA) cell counting‐slide, hence in absence of conventional high‐reflectivity optical cavities. The exceptional spectral narrowing and the steep slope increase in the input–output energy diagram prove occurrence of laser action in presence of cells. The observed biolasing is an intrinsically dynamic signal, with large fluctuations in intensity and spectrum determined by the optical properties of the individual cell passing through the pump beam. Numerical simulations of the scattering efficiency rule out the possibility of optical feedback from either WGM (whispering gallery mode) or multiple scattering within the cell, and point to the enhanced directional scattering field as the crucial contribution of cells to the laser action. ... mehr

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Verlagsausgabe §
DOI: 10.5445/IR/1000105522
Veröffentlicht am 16.07.2020
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Nanotechnologie (INT)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2020
Sprache Englisch
Identifikator ISSN: 2195-1071, 2195-1071
KITopen-ID: 1000105522
HGF-Programm 43.21.04 (POF III, LK 01)
Molecular Engineering
Erschienen in Advanced optical materials
Band 8
Heft 6
Seiten Article: 1901573
Vorab online veröffentlicht am 24.01.2020
Nachgewiesen in Scopus
Web of Science
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