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Development of immunoassays for the investigation of peptide antibody interactions in array format

Palermo, Andrea

Abstract (englisch):
Antibodies perform a variety of important tasks in the immune system to protect us from diseases. They are trained to specifically target pathogens, which might be toxins, bacteria or viruses. However, they can also harm us by erroneously mark endogenous structures for degradation, which contributes to autoimmune diseases. Studying the immune response by determining, which antibodies are amplified during a successful immune reaction, and which part of the pathogen neutralizing antibodies are binding to, would help to develop new vaccine candidates and contribute to the common knowledge of the underlying mechanism. In addition to the information, what antibodies specifically bind to, it is of interest, in which concentration they are available in the blood and with which affinity they bind their target. Peptide arrays provide an ideal platform for the study of antibody-protein interactions, with the peptides mimicking antibody targets. Using a minute sample volume, several thousand peptides can be examined simultaneously. To study identified antibody-peptide interactions in terms of kinetics, on-line measurements in microfluidic channels, so called continuous flow assays, have to be performed. ... mehr

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Volltext §
DOI: 10.5445/IR/1000105997
Veröffentlicht am 03.03.2020
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Mikrostrukturtechnik (IMT)
Publikationstyp Hochschulschrift
Publikationsdatum 03.03.2020
Sprache Englisch
Identifikator KITopen-ID: 1000105997
Verlag Karlsruher Institut für Technologie (KIT)
Umfang XII, 135 S.
Art der Arbeit Dissertation
Fakultät Fakultät für Maschinenbau (MACH)
Institut Institut für Mikrostrukturtechnik (IMT)
Prüfungsdatum 03.12.2019
Referent/Betreuer Prof. A. Nesterov-Müller
Schlagwörter peptide array; peptide-antibody interaction; microfluidic channel; immunoassay
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