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Synergistic Treatment of Tumor by Targeted Biotherapy and Chemotherapy via Site-Specific Anchoring of Aptamers on DNA Nanotubes

Chen, Run; Sun, Pengchao; Chu, Xiao; Pu, Xiaorong; Yang, Yang; Zhang, Nan; Zhao, Yongxing

Abstract:
Background: Aptamers have been widely used as targeted therapeutic agents due to its relatively small physical size, flexible structure, high specificity, and selectivity. Aptamers functionalized nanomaterials, not only enhance the targeting of nanomaterials, but can also improve the stability of the aptamers. We developed aptamer C2NP (Apt) conjugated straight DNA nanotubes (S-DNT-Apt) and twisted DNA nanotubes (T-DNT-Apt) as nanocarriers for doxorubicin (DOX).
Methods: The twisted DNA nanotubes (T-DNT) and straight DNA nanotubes (S-DNT) were assembled with a scaffold and hundreds of staples. Apt was site-specifically anchored on DNA nanotubes with either different spatial distribution (3 or 6 nm) or varied stoichiometry (15Apt or 30Apt). The developed nanocarriers were characterized with agarose gel electrophoresis and transmission electron microscopy. The drug loading and release in vitro were evaluated by measuring the fluorescence intensity of DOX using a microplate reader. The stability of DNT in cell culture medium plus 10% of FBS was evaluated by agarose gel electrophoresis. The cytotoxicity of DNA nanostructures against K299 cells was tested with a standard CCK8 method. ... mehr

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Verlagsausgabe §
DOI: 10.5445/IR/1000117794
Veröffentlicht am 19.03.2020
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Biologische Grenzflächen (IBG)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2020
Sprache Englisch
Identifikator ISSN: 1178-2013
KITopen-ID: 1000117794
HGF-Programm 47.02.01 (POF III, LK 01)
Zellpopul.auf Biofunk.Oberflächen IBG-1
Erschienen in International journal of nanomedicine
Band Volume 15
Seiten 1309–1320
Vorab online veröffentlicht am 27.02.2020
Schlagwörter DNA nanotubes, aptamers, controlled spatial distribution, biotherapy and chemotherapy
Nachgewiesen in Scopus
Web of Science
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