A comprehensive literature review is conducted to create a new database of malaria Entomological Inoculation Rates (EIR), a metric of malaria transmission intensity. All field studies include data at a monthly temporal resolution and a duration of at least one year in order to be able to study seasonality variability in transmission. Care is taken to ensure the data collection methodologies adhere to a specific standard and that the location and timing of the measurements are well documented. Auxiliary information on the population and hydrological setting are included. The database includes measurements that cover Sub-Saharan Africa spanning from 1968 to 2013, thus also facilitating analysis of interannual transmission variability over broad regions.