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Tools to Ease the Choice and Design of Protein Crystallisation Experiments

Rosa, Nicholas; Ristic, Marko; Thorburn, Luke; Abrahams, Gabriel J.; Marshall, Bevan; Watkins, Christopher J.; Kruger, Alex; Khassapov, Alex; Newman, Janet

Abstract:
The process of macromolecular crystallisation almost always begins by setting up crystallisation trials using commercial or other premade screens, followed by cycles of optimisation where the crystallisation cocktails are focused towards a particular small region of chemical space. The screening process is relatively straightforward, but still requires an understanding of the plethora of commercially available screens. Optimisation is complicated by requiring both the design and preparation of the appropriate secondary screens. Software has been developed in the C3 lab to aid the process of choosing initial screens, to analyse the results of the initial trials, and to design and describe how to prepare optimisation screens.

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Verlagsausgabe §
DOI: 10.5445/IR/1000118066
Veröffentlicht am 03.04.2020
Originalveröffentlichung
DOI: 10.3390/cryst10020095
Scopus
Zitationen: 1
Web of Science
Zitationen: 1
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Anthropomatik und Robotik (IAR)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2020
Sprache Englisch
Identifikator ISSN: 2073-4352
KITopen-ID: 1000118066
Erschienen in Crystals
Verlag MDPI
Band 10
Heft 2
Seiten Article: 95
Vorab online veröffentlicht am 07.02.2020
Schlagwörter crystallization cocktail; crystallization screen; crystallization optimisation; similarity
Nachgewiesen in Scopus
Web of Science
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