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Evaluation of Downstream Processing, Extraction, and Quantification Strategies for Single Cell Oil Produced by the Oleaginous Yeasts Saitozyma podzolica DSM 27192 and Apiotrichum porosum DSM 27194

Gorte, Olge; Hollenbach, Rebecca; Papachristou, Ioannis; Steinweg, Christian; Silve, Aude; Frey, Wolfgang; Syldatk, Christoph; Ochsenreither, Katrin

Abstract (englisch):
Single cell oil (SCO) produced by oleaginous yeasts is considered as a sustainable source for biodiesel and oleochemicals since its production does not compete with food or feed and high yields can be obtained from a wide variety of carbon sources, e.g., acetate or lignocellulose. Downstream processing is still costly preventing the
broader application of SCO. Direct transesterification of freeze-dried biomass is widely used for analytical purposes and for biodiesel production but it is energy intensive and, therefore, expensive. Additionally, only fatty acid esters are produced limiting the subsequent applications. The harsh conditions applied during direct esterification might also damage high-value polyunsaturated fatty acids. Unfortunately, universal downstream strategies effective for all yeast species do not exist and methods have to be developed for each yeast species due to differences in cell wall composition.
Therefore, the aim of this study was to evaluate three industrially relevant cell disruption methods combined with three extraction systems for the SCO extraction of two novel, unconventional oleaginous yeasts, Saitozyma podzolica DSM 27192 and Apiotrichum porosum DSM 27194, based on cell disruption efficiency, lipid yield, and oil quality.
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Verlagsausgabe §
DOI: 10.5445/IR/1000118670
Veröffentlicht am 03.06.2020
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Bio- und Lebensmitteltechnik (BLT)
Institut für Hochleistungsimpuls- und Mikrowellentechnik (IHM)
Publikationstyp Zeitschriftenaufsatz
Publikationsdatum 24.04.2020
Sprache Englisch
Identifikator ISSN: 2296-4185
KITopen-ID: 1000118670
HGF-Programm 35.13.01 (POF III, LK 01)
Biomassen und Vorbehandlung
Erschienen in Frontiers in Bioengineering and Biotechnology
Band 8
Seiten Article no: 355
Schlagwörter single cell oil, oleaginous yeasts, downstream processing, cell disruption, lipid extraction,transesterification, Saitozyma podzolica DSM 27192, Apiotrichum porosum DSM 27194
Nachgewiesen in Scopus
Web of Science
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