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Phosphoprotein Detection with a Single Nanofluidic Diode Decorated with Zinc Chelates

Nasir, S.; Ali, M.; Ahmed, I.; Niemeyer, C. M.; Ensinger, W.

Abstract (englisch):
We report a nanofluidic device for the label‐free detection of phosphoprotein (PPn) analytes. To achieve this goal, a metal ion chelator, namely 4‐[bis(2‐pyridylmethyl)aminomethyl]aniline (DPA−NH2) compound was synthesized. Single asymmetric nanofluidic channels were fabricated in polyethylene terephthalate (PET) membranes. The chelator (DPA−NH2) molecules are subsequently immobilized on the nanochannel surface, followed by the zinc ion complexation to afford DPA−Zn2+ chelates, which act as ligand moieties for the specific binding of phosphoproteins. The success of the chemical reaction and biomolecular recognition process that occur in a confined geometry can be monitored from the changes in electrical readout of the nanochannel. The nanofluidic sensor has the ability to sensitively and specifically detect lower concentrations (≥1 nM) of phosphoprotein (albumin and α‐casein) in the surrounding environment as evidenced from the significant decrease in ion current flowing through the nanochannels. However, dephosphoproteins such as lysozyme and dephospho‐α‐casein even at higher concentration (>1 μM) could not induce any significant change in the transmembrane ion flux. ... mehr

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Verlagsausgabe §
DOI: 10.5445/IR/1000118846
Veröffentlicht am 05.05.2020
DOI: 10.1002/cplu.202000045
Zitationen: 2
Web of Science
Zitationen: 2
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Biologische Grenzflächen (IBG)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2020
Sprache Englisch
Identifikator ISSN: 2192-6506
KITopen-ID: 1000118846
HGF-Programm 47.02.01 (POF III, LK 01) Zellpopul.auf Biofunk.Oberflächen IBG-1
Erschienen in ChemPlusChem
Verlag Wiley
Band 85
Heft 3
Seiten 587-594
Vorab online veröffentlicht am 17.02.2020
Nachgewiesen in Web of Science
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