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A survey of extended-spectrum beta-lactamase-producing Enterobacteriaceae in urban wetlands in southwestern Nigeria as a step towards generating prevalence maps of antimicrobial resistance

Adelowo, Olawale Olufemi; Ikhimiukor, Odion Osebhahiemen; Knecht, Camila; Vollmers, John; Bhatia, Mudit; Kaster, Anne-Kirstin; Müller, Jochen A.

Abstract (englisch):
In many countries, emission of insufficiently treated wastewater into water bodies appears to be an important factor in spreading clinically relevant antimicrobial resistant bacteria. In this study, we looked for the presence of Enterobacteriaceae strains with resistance to 3rd generation cephalosporin antibiotics in four urban wetlands in southwestern Nigeria by isolation, whole genome sequencing and qPCR enumeration of marker genes. Genome analysis of multi-drug resistant and potentially pathogenic Escherichia coli isolates (members of the widely distributed ST10 complex) revealed the presence of the extended spectrum beta-lactamase gene blaCTX-M-15 on self-transmissible IncF plasmids. The gene was also present together with a blaTEM-1B gene on self-transmissible IncH plasmids in multi-drug resistant Enterobacter cloacae isolates. A Citrobacter freundii isolate carried blaTEM-1B on an IncR-type plasmid without discernable conjugation apparatus. All strains were isolated from a wetland for which previous qPCR enumeration of marker genes, in particular the ratio of intI1 to 16S rRNA gene copy numbers, had indicated a strong anthropogenic impact. ... mehr

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Verlagsausgabe §
DOI: 10.5445/IR/1000118906
Veröffentlicht am 04.05.2020
DOI: 10.1371/journal.pone.0229451
Zitationen: 2
Web of Science
Zitationen: 2
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Biologische Grenzflächen (IBG)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2020
Sprache Englisch
Identifikator ISSN: 1932-6203
KITopen-ID: 1000118906
HGF-Programm 47.02.05 (POF III, LK 01) Zellpopul.auf Biofunk.Oberflächen IBG-5
Erschienen in PLOS ONE
Verlag Public Library of Science (PLoS)
Band 15
Heft 3
Seiten Article No.e0229451
Vorab online veröffentlicht am 04.03.2020
Nachgewiesen in Scopus
Web of Science
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