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Lipid ratios as a marker for red blood cell storage quality and as a possible explanation for donor gender differences in storage quality [in press]

Melzak, Kathryn A.; Muth, Marius; Kirschhöfer, Frank; Brenner‐Weiss, Gerald; Bieback, Karen

Abstract:
Background and objectives

Red blood cells that are stored for transfusions as red cell concentrates (RCCs) undergo changes during the storage period, culminating in the lysis of the cells. The goal of this work is to find markers that are linked to high haemolysis, in order to explain the inter‐donor variability that is known to occur in storage quality, and also the known differences between RCCs from male and female donors.


Materials and methods

The relative amounts of lipids at the end of the storage period were compared for one group of low haemolysis samples (24 units, all ≤0·15% haemolysis), and one group of high haemolysis samples (26 units, all ≥0·5% haemolysis). Representative lipids were analysed from different lipid classes, including cholesterol, phosphatidylcholine, phosphatidylethanolamine, sphingomyelin and ceramide. Whole membrane preparations were analysed with one mass spectrometry technique, and lipid extracts were analysed with a second mass spectrometry technique.


Results

The ratio of palmitoyl‐oleoyl phosphatidylcholine (POPC) to sphingomyelin was different for the high and low haemolysis groups (P = 0·0001) and for the RCCs from male and female donors (P = 0·0009). ... mehr

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Zugehörige Institution(en) am KIT Institut für Funktionelle Grenzflächen (IFG)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2020
Sprache Englisch
Identifikator ISSN: 0042-9007, 1423-0410
KITopen-ID: 1000119462
Erschienen in Vox sanguinis
Vorab online veröffentlicht am 06.05.2020
Nachgewiesen in Web of Science
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