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Separation and degradation detection of nanogram-per-litre concentrations of radiolabelled steroid hormones using combined liquid chromatography and flow scintillation analysis

Lyubimenko, R.; Richards, B. S.; Turshatov, A.; Schäfer, A. I.

Abstract:
Detection of micropollutants such as steroid hormones occurring in the aquatic environment at concentrations between ng/L and µg/L remains a major challenge, in particular when treatment efficiency is to be evaluated. Steroid hormones are typically analysed using mass-spectrometry methods, requiring pre-concentration and/or derivatisation procedures to achieve required detection limits. Free of sample preparation steps, the use of radiolabelled contaminants with liquid scintillation counting is limited to single-compound systems and require a separation of hormone mixtures before detection. In this work, a method was developed coupling ultra-high-pressure liquid chromatography (UHPLC) with flow scintillation analysis (FSA) for separation and detection of radiolabelled estrone, 17ß-estradiol, testosterone and progesterone. Adjustment of the flow rate of scintillation liquid and UHPLC mobile phase, gradient time, column temperature, and injection volume allowed the separation of steroid hormones and degradation products. The limit-of-detection (LOD = 1.5–2.4 ng/L) and limit-of-quantification (LOQ = 3.4–4.3 ng/L) for steroid hormones were comparable with the current state-of-the-art technique (LC-MS/MS) for non-derivatised compounds. ... mehr

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Verlagsausgabe §
DOI: 10.5445/IR/1000119567
Veröffentlicht am 16.06.2020
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Mikrostrukturtechnik (IMT)
Lichttechnisches Institut (LTI)
Institute for Advanced Membrane Technology (IAMT)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2020
Sprache Englisch
Identifikator ISSN: 2045-2322
KITopen-ID: 1000119567
Erschienen in Scientific reports
Band 10
Heft 1
Seiten Article: 7095
Nachgewiesen in Scopus
Web of Science
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