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An NMR-Based Approach to Identify Urinary Metabolites Associated with Acute Physical Exercise and Cardiorespiratory Fitness in Healthy Humans—Results of the KarMeN Study

Kistner, Sina; Rist, Manuela J.; Döring, Maik; Dörr, Claudia; Neumann, Rainer; Härtel, Sascha; Bub, Achim

Abstract (englisch):
Knowledge on metabolites distinguishing the metabolic response to acute physical
exercise between fit and less fit individuals could clarify mechanisms and metabolic pathways contributing to the beneficial adaptations to exercise. By analyzing data from the cross-sectional KarMeN (Karlsruhe Metabolomics and Nutrition) study, we characterized the acute effects of a
standardized exercise tolerance test on urinary metabolites of 255 healthy women and men. In a second step, we aimed to detect a urinary metabolite pattern associated with the cardiorespiratory fitness (CRF), which was determined by measuring the peak oxygen uptake (VO2peak) during incremental exercise. Spot urine samples were collected pre- and post-exercise and 47 urinary metabolites were identified by nuclear magnetic resonance (NMR) spectroscopy. While the
univariate analysis of pre-to-post-exercise differences revealed significant alterations in 37 urinary metabolites, principal component analysis (PCA) did not show a clear separation of the pre- and post-exercise urine samples. Moreover, both bivariate correlation and multiple linear regression analyses revealed only weak relationships between the VO2peak and single urinary metabolites or
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Verlagsausgabe §
DOI: 10.5445/IR/1000119778
Veröffentlicht am 02.07.2020
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Sport und Sportwissenschaft (IfSS)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2020
Sprache Englisch
Identifikator ISSN: 2218-1989
KITopen-ID: 1000119778
Erschienen in Metabolites
Band 10
Heft 5
Seiten Art.Nr. 212
Vorab online veröffentlicht am 21.05.2020
Schlagwörter metabolomics; urinary metabolome; urinary metabolites; NMR spectroscopy; acute physical exercise; cardiorespiratory fitness; VO2peak; exercise metabolomics
Nachgewiesen in Web of Science
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