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Simulative Minimization of Mass Transfer Limitations Within Hydrogel-Based 3D-Printed Enzyme Carriers

Schmieg, B. 1; Nguyen, M. 1; Franzreb, M. 1
1 Institut für Funktionelle Grenzflächen (IFG), Karlsruher Institut für Technologie (KIT)


In biotechnology, immobilization of functional reactants is often done as a surface immobilization on small particles. Examples are chromatography columns and fixedbed reactors. However, the available surface for immobilization is directly linked to particle diameter and bed porosity for these systems, leading to high backpressure for small particle sizes. When larger molecules, such as enzymes are immobilized, physical entrapment within porous materials like hydrogels is an alternative. An emerging technique for the production of geometrically structured, three-dimensional and scalable hollow bodies is 3D-printing. Different bioprinting methods are available to produce structures of the desired size, resolution and solids content. However, in case of entrapped enzymes mass transfer limitations often determine the achievable reactivities. With increasing complexity of the system, for example a fixed-bed reactor, 3D-simulation is indispensable to understand the local reaction conditions to be able to highlight the optimization potential. Based on experimental data, this manuscript shows the application of the dimensionless numbers effectiveness factor and Thiele modulus for the design of a 3D-printed flow-through reactor. ... mehr

Verlagsausgabe §
DOI: 10.5445/IR/1000119892
Veröffentlicht am 29.05.2020
DOI: 10.3389/fbioe.2020.00365
Zitationen: 10
Web of Science
Zitationen: 8
Zitationen: 10
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Funktionelle Grenzflächen (IFG)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2020
Sprache Englisch
Identifikator ISSN: 2296-4185
KITopen-ID: 1000119892
HGF-Programm 47.02.06 (POF III, LK 01) Zellpopul.auf Biofunk.Oberflächen IFG
Erschienen in Frontiers in Bioengineering and Biotechnology
Verlag Frontiers Media SA
Band 8
Seiten 365
Vorab online veröffentlicht am 28.04.2020
Schlagwörter enzyme immobilization, hydrogel, mass transfer limitation, effectiveness factor, Thiele modulus, 3D-printing
Nachgewiesen in Dimensions
Web of Science
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