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Chemical Engineering of Plants for Salt Tolerance

Asfaw, Kinfemichael Geressu

Salinity is one of the serious threats to global agriculture that threatens human food security. To tackle the problem, there were and still are quite several scientific efforts in place. Hence, in this dissertation, we used chemical and genetic engineering approaches to unravel the sophisticated knot of the problem using a Trojan peptoid called plant PeptoQ and overexpression of OsOPR7 gene in non-transformed WT tobacco BY-2 cells. First, plant PeptoQ that can be used to target a functional cargo (a rhodamine-labelled semiquinone peptoid as mimetic of coenzyme Q10) into mitochondria of tobacco BY-2 cells was characterized with regard to its cellular uptake and potential cytotoxicity. We found that the uptake is specific for mitochondria, rapid, dose-dependent, and requires both clathrin-mediated and clathrin-independent endocytosis, as well as actin filaments, while microtubules seem to be dispensable. Viability of the treated cells was not affected, and they showed better survival under salt stress, a condition that perturbs oxidative homeostasis in mitochondria. Using double labelling with appropriate fluorescent markers, we showed that targeting of this Trojan Peptoid to the mitochondria is not based on a passage through the plasma membrane (as thought hitherto), but on import via endocytotic vesicles and subsequent accumulation of the positively charged side chains at the negatively charged inner mitochondrial membrane. ... mehr

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DOI: 10.5445/IR/1000119921
Veröffentlicht am 03.06.2020
Cover der Publikation
Zugehörige Institution(en) am KIT Botanisches Institut (BOTANIK)
Publikationstyp Hochschulschrift
Publikationsdatum 03.06.2020
Sprache Englisch
Identifikator KITopen-ID: 1000119921
Verlag Karlsruher Institut für Technologie (KIT)
Umfang XVII, 152 S.
Art der Arbeit Dissertation
Fakultät Fakultät für Chemie und Biowissenschaften (CHEM-BIO)
Institut Botanisches Institut (BOTANIK)
Prüfungsdatum 05.02.2020
Referent/Betreuer Prof. P. Nick
KIT – Die Forschungsuniversität in der Helmholtz-Gemeinschaft
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