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Segregation of Dispersed Silica Nanoparticles in Microfluidic Water‐in‐Oil Droplets: A Kinetic Study

Sheshachala, Sahana; Grösche, Maximilian; Scherr, Tim; Hu, Yong; Sun, Pengchao; Bartschat, Andreas; Mikut, Ralf; Niemeyer, Christof M.

Dispersed negatively charged silica nanoparticles segregate inside microfluidic water-in-oil (W/O) droplets that are coated with a positively charged lipid shell. We report a methodology for the quantitative analysis of this self-assembly process. By using real-time fluorescence microscopy and automated analysis of the recorded images, kinetic data are obtained that characterize the electrostatically-driven self-assembly. We demonstrate that the segregation rates can be controlled by the installment of functional moieties on the nanoparticle’s surface, such as nucleic acid and protein molecules. We anticipate that our method enables the quantitative and systematic investigation of the segregation of (bio)functionalized nanoparticles in microfluidic droplets. This could lead to complex supramolecular architectures on the inner surface of micrometer-sized hollow spheres, which might be used, for example, as cell containers for applications in the life sciences.

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Verlagsausgabe §
DOI: 10.5445/IR/1000119996
Veröffentlicht am 05.06.2020
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Biologische Grenzflächen (IBG)
Institut für Automation und angewandte Informatik (IAI)
Publikationstyp Zeitschriftenaufsatz
Publikationsmonat/-jahr 05.2020
Sprache Englisch
Identifikator ISSN: 1439-4235, 1439-7641
KITopen-ID: 1000119996
HGF-Programm 47.02.01 (POF III, LK 01)
Zellpopul.auf Biofunk.Oberflächen IBG-1
Erschienen in ChemPhysChem
Band 21
Heft 10
Seiten 1070–1078
Vorab online veröffentlicht am 06.03.2020
Nachgewiesen in Web of Science
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